Illumina MiSeq and IonTorrent PGM Technology and Price Comparison Multiple studies and groups have compared the performance of current next generation sequencing (NGS) technologies. Independent comparisons of different NGS sequencing machines have given us a good view of what one can expect from these constantly changing technologies. Apart from performance, it is also good to […]
I love PCR – and who doesn’t? A fantastic inforgraphic from Life Technologies exploring PCR – enjoy! References Guyer RL and Koshland DL (1989) “The Molecule of the Year.” Science 246:1543–1546. Saiki RK, et al. (1985) “Enzymatic amplification of β-globin genomic sequences and restriction site analysis for sickle cell anemia” Science 230:1350–1354. Saiki RK, Gelfand DH, […]
Using Cell-free DNA as a Biomarker The potential uses of cfDNA as a biomarker are especially relevant to NSCLC due to the difficulties of collecting sufficient tumor tissue. This has stimulated interest in analyses using serum and plasma samples which contain cell-free DNA originating from tumor tissues. Detecting somatic mutations from plasma DNA in advanced […]
What are Circulating Tumor Cells (CTCs)? I often get asked what are CTCs (also known as Cell-Free DNA (cfDNA)), JAMA recently published a great article that explains it very nicely. Enjoy!
Personalized medicine, with the aid of molecular diagnostics, is providing the exciting possibility of cost-effective tailored therapies, based on an individual patient’s genetic code. This is particularly true in the case of cancer where a single nucleotide polymorphism (SNP) out of a three billion-base genome can be the difference between having, and not having, an […]