Illumina MiSeq and IonTorrent PGM Technology and Price Comparison
Apart from performance, it is also good to know about price and tech. spec. differences – a much easier aspects of NGS sequencing technology comparisons. As smaller labs have started thinking about using NGS sequencing, learning about the costs and tech specs of different sequencing technologies is often the first step. Loman et. al and Quail et. al. have presented a nice comparison of price and technical specifications for the current NGS sequencing machines by sequencing bacterial genomes.
Both Loman et. al and Quail et. al compared the NGS sequencing technologies available as of Dec. 2011. Indeed, some aspects of the price comparison does differ and will be slightly different. Here is a brief summary of the pricing and sequencing capability comparisons done by Loman et. al. and Quail. et. al.
Loman et. al. performed a detailed comparison of 454 GS Junior, Ion Torrent PGM, and Illumina MiSeq- the current benchtop next-gen sequencers by sequencing E.Coli.
PB output per cell these days is 750 Mb to 1 Gb with P6-C4 chemistry. After sample prep costs, the cost per cell varies based on the core lab and locale but you could estimate it by taking a lower cost service provider (e.g. Duke at https://dugsim.net/estimate_cost) and dividing by 2 to account for their labor. Should be closer to $160 / Gb now per cell, depending upon the project.
According the PB website (http://www.pacb.com/wp-content/uploads/2015/09/PacBio_RS_II_Brochure.pdf): Data per SMRT Cell: 500 Mb – 1 Gb on RSII system. It is like 6th web comparison of ngs platform I ve checked today and not even one put there ANY references.
Good job.
Regards for helping out, good info.