Genetic tests could actually increase the price of medical care It’s exactly 62 years since James Watson and Francis Crick described the structure of DNA and 12 years since the first sequence of the genome and as I write this blog I’m sitting in the ‘supposed’ pub where it was discovered in Cambridge. The first […]
….no, not the French word for fish. Poisson distribution is a mathematical principle that becomes incredibly important at low levels. So why am I blogging about it? Well these ‘low levels’ can be found in cell free DNA (cfDNA) and copy number variants (CNV) due to the ‘dilution principle’. Although most pathologists would be more […]
Should I use Nanodrop or Qubit? The DNA quantification dilemma. Many laboratories often ask whether they should be using Nanodrop or Qubit. Both with their own pro’s and con’s which can be nicely summarized in the table below. Qubit Fluorometer NanoDrop ND-1000 Quantification method Fluorescence-based dyes that bind specifically to DNA, RNA, or protein UV absorbance […]
Serial monitoring of circulating tumor DNA in patients with primary breast cancer for detection of occult metastatic disease Lao Saal, M.D, Ph.D., Head of the Translational Oncogenomics Unit The potential for cell free DNA to be used as biomarkers for prognostic, predictive and diagnostic means are great and a fantastic recent publication by Saal […]
Illumina MiSeq and IonTorrent PGM Technology and Price Comparison Multiple studies and groups have compared the performance of current next generation sequencing (NGS) technologies. Independent comparisons of different NGS sequencing machines have given us a good view of what one can expect from these constantly changing technologies. Apart from performance, it is also good to […]
I love PCR – and who doesn’t? A fantastic inforgraphic from Life Technologies exploring PCR – enjoy! References Guyer RL and Koshland DL (1989) “The Molecule of the Year.” Science 246:1543–1546. Saiki RK, et al. (1985) “Enzymatic amplification of β-globin genomic sequences and restriction site analysis for sickle cell anemia” Science 230:1350–1354. Saiki RK, Gelfand DH, […]
What are Circulating Tumor Cells (CTCs)? I often get asked what are CTCs (also known as Cell-Free DNA (cfDNA)), JAMA recently published a great article that explains it very nicely. Enjoy!